Read number effect
Here shows analysis of sample size effect
Samples
The following three metagenomic data were used for analysis.
・Hot spling [DRR163688]: reported temperature is 96.8°C
・Human gut [DRR127552]
・Soil (main fire)[SRR7882662]: reported temperature is 57.4°C
Subsampling
Arbitrary number of reads were subsampled by 30 replicates using Seqkit.
Graph summarized the MPT predicted from each subsamples.
Conclusion
When the read number is more than 100,000, standard deviation of the predicted value can be suppressed to 0.5°C or less in various environmental data.